Association of Nanodiamond Rotation Dynamics with Cell Activities by Translation-Rotation Tracking
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2021-04-13 |
| Journal | Nano Letters |
| Authors | Xi Feng, Weng-Hang Leong, Kangwei Xia, Chufeng Liu, GangāQin Liu |
| Institutions | Chinese University of Hong Kong, University of Stuttgart |
| Citations | 31 |
Abstract
Section titled āAbstractāCorrelated translation-orientation tracking of single particles can provide important information for understanding the dynamics of live systems and their interaction with the probes. However, full six-dimensional (6D) motion tracking has yet to be achieved. Here, we developed synchronized 3D translation and 3D rotation tracking of single diamond particles based on nitrogen-vacancy center sensing. We first performed 6D tracking of diamond particles attached to a giant plasma membrane vesicle to demonstrate the method. Quantitative analysis of diamond particlesā motion allowed elimination of the geometric effect and revealed the net rotation on the vesicle. 6D tracking was then applied to measure live cell dynamics. Motion characteristics of nanodiamonds on cell membranes under various controlled physiological conditions suggest that the nanodiamondsā rotation is associated with cell metabolic activities. Our technique extends the toolbox of single particle tracking and provides a unique solution to problems where correlated analysis of translation and rotation is critical.