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6CCVD Research

What Are the Key Factors for the Detection of Peptides Using Mass Spectrometry on Boron-Doped Diamond Surfaces?

At a Glance

Section titled ā€œAt a Glanceā€
MetadataDetails
Publication Date2024-07-24
JournalNanomaterials
AuthorsJuvissan Aguedo, MariĆ”n Vojs, Martin VrÅ”ka, Marek Nemčovič, Zuzana PakanovĆ”
InstitutionsInstitute of Chemistry of the Slovak Academy of Sciences, Slovak Academy of Sciences

Abstract

Section titled ā€œAbstractā€

We investigated the use of boron-doped diamond (BDD) with different surface morphologies for the enhanced detection of nine different peptides by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). For the first time, we compared three different nanostructured BDD film morphologies (Continuous, Nanograss, and Nanotips) with differently terminated surfaces (-H, -O, and -F) to commercially available Ground Steel plates. All these surfaces were evaluated for their effectiveness in detecting the nine different peptides by MALDI-MS. Our results demonstrated that certain nanostructured BDD surfaces exhibited superior performance for the detection of especially hydrophobic peptides (e.g., bradykinin 1-7, substance P, and the renin substrate), with a limit of detection of down to 2.3 pM. Further investigation showed that hydrophobic peptides (e.g., bradykinin 1-7, substance P, and the renin substrate) were effectively detected on hydrogen-terminated BDD surfaces. On the other hand, the highly acidic negatively charged peptide adrenocorticotropic hormone fragment 18-39 was effectively identified on oxygen-/fluorine-terminated BDD surfaces. Furthermore, BDD surfaces reduced sodium adduct contamination significantly.

Tech Support

Section titled ā€œTech Supportā€

Original Source

Section titled ā€œOriginal Sourceā€

References

Section titled ā€œReferencesā€
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  2. 2022 - MALDI-TOF Mass Spectrometry in Clinical Analysis and Research [Crossref]
  3. 2023 - Identification of Conformational Variants for Bradykinin Biomarker Peptides from a Biofluid Using a Nanopore and Machine Learning [Crossref]
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  6. 2018 - Multifunctional Au NPs-polydopamine-polyvinylidene fluoride membrane chips as probe for enrichment and rapid detection of organic contaminants [Crossref]
  7. 2019 - Ag nanoparticles/ZnO nanorods for highly sensitive detection of small molecules with laser desorption/ionization mass spectrometry [Crossref]
  8. 2019 - Magnetic metal-organic framework nanocomposites for enrichment and direct detection of environmental pollutants by negative-ion matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [Crossref]

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