P2EC.20 - Electrochemical behavior and nanomolar quantification of polydatin using boron doped diamond electrode
At a Glance
Section titled âAt a Glanceâ| Metadata | Details |
|---|---|
| Publication Date | 2018-01-01 |
| Journal | Proceedings IMCS 2018 |
| Authors | Dalibor StankoviÄ, Vesna VukojeviÄ, Sladjana DjurdjiÄ, Aleksandar VukadinoviÄ, MiloĹĄ OgnjanoviÄ |
| Institutions | University of Belgrade, Institute for Technology of Nuclear and other Mineral Raw Materials |
| Analysis | Full AI Review Included |
Electrochemical Polydatin Quantification: Boron Doped Diamond (BDD) Analysis
Section titled âElectrochemical Polydatin Quantification: Boron Doped Diamond (BDD) AnalysisâExecutive Summary
Section titled âExecutive SummaryâThis research validates the superior performance of Boron Doped Diamond (BDD) electrodes for highly sensitive, nanomolar quantification of complex natural compounds (polydatin) in pharmaceutical applications. The key findings and value proposition for engineers leveraging MPCVD diamond technology are:
- Ultra-Low Detection Limits: Achieved a Limit of Detection (LOD) of 6 x 10-9 M, demonstrating the high sensitivity and minimal residual current inherent in sp3 hybridized diamond electrodes.
- Irreversible Oxidation Process: Polydatin oxidation exhibited irreversible electrochemical behavior with sharp, well-defined anodic peaks at 0.73 V and 1.3 V, confirming BDD stability across a wide potential range (-1.5 V to 2 V).
- Diffusion-Controlled Mechanism: The dominant process at low concentrations (1 x 10-7 to 9 x 10-6 M) was confirmed to be diffusion-controlled, indicating minimal absorption interference on the BDD surface.
- Material Advantages Confirmed: The method relies entirely on the recognized benefits of BDD, including high signal/noise ratio, excellent mechanical and chemical stability, and broad operational pH stability.
- Pharmaceutical Quantification: The optimized Square Wave Voltammetry (SWV) method successfully quantified polydatin in commercial POLIDAL tablets, proving BDDâs robustness for complex sample matrices.
- 6CCVD Relevance: The specialized BDD material (1000 ppm boron doping, 3 mm diameter) is a core offering of 6CCVD, enabling direct replication or optimization of this high-precision sensor design.
Technical Specifications
Section titled âTechnical SpecificationsâThe following hard data points were extracted detailing the BDD electrode characteristics and analytical performance achieved in the study:
| Parameter | Value | Unit | Context |
|---|---|---|---|
| Electrode Material | Boron Doped Diamond (BDD) | N/A | Working electrode |
| BDD Diameter | 3 | mm | Specific dimension used for the cell |
| Boron Doping Level | 1000 | ppm | Targeted specification of the BDD film |
| Electrochemical Cell Type | Three-electrode | N/A | Conventional setup (BDD, Ag/AgCl, Pt wire) |
| Operational Potential Range | -1.5 to 2 | V | Full range tested via Cyclic Voltammetry |
| Scan Rate (CV) | 100 | mV/s | Rate used for behavioral analysis |
| Primary Oxidation Peak (Epa1) | 0.73 | V | Well-defined peak used for SWV quantification |
| Secondary Oxidation Peak (Epa2) | 1.3 | V | Wider peak observed |
| Optimal Supporting Electrolyte pH | 2 | N/A | BRBS (Britton-Robinson Buffer Solution) |
| Limit of Detection (LOD) | 6 x 10-9 | M | Ultra-low concentration detection limit |
| Limit of Quantification (LOQ) | 2 x 10-8 | M | Minimal concentration for reliable measurement |
| Low Concentration Linear Range | 1 x 10-7 to 9 x 10-6 | M | Diffusion-controlled region (R=0.9977) |
| High Concentration Linear Range | 2 x 10-5 to 7 x 10-5 | M | Adsorption-controlled region (R=0.9961) |
Key Methodologies
Section titled âKey MethodologiesâThe polydatin quantification was achieved using precise electrochemical techniques optimized for the BDD material:
- Electrode Setup: A conventional three-electrode cell was employed using a potentiostat/galvanostat (CHI 760b).
- Working Electrode: Boron Doped Diamond (BDD) (3 mm diameter, 1000 ppm Boron).
- Reference Electrode: 3M KCl Ag/AgCl.
- Auxiliary (Counter) Electrode: Platinum wire of large surface area.
- Potential Range Selection: Initial electrochemical behavior was mapped across a wide potential window (-1.5 V to 2 V) using Cyclic Voltammetry (CV) at 100 mV/s.
- pH Optimization: The influence of pH (range 1 to 9) on peak potential and current was studied to determine the optimal conditions for maximum signal resolution (determined to be pH 2).
- Quantification Method: Quantification was performed using Square Wave Voltammetry (SWV) focused on the first oxidation peak (0.73 V), providing enhanced sensitivity over CV.
- Linear Range Determination: Two distinct linear working ranges were established, reflecting concentration-dependent mechanisms:
- Low Concentration: Diffusion control (10-7 M to 10-6 M range).
- High Concentration: Adsorption phenomenon (10-5 M range).
- Pharmaceutical Application: The optimized SWV method was applied directly to quantify polydatin content in commercial POLIDAL tablets.
6CCVD Solutions & Capabilities
Section titled â6CCVD Solutions & Capabilitiesâ6CCVD provides the specialized, high-purity diamond required to replicate, improve, and industrialize the BDD-based sensor technology detailed in this research. Our capabilities ensure that the critical performance metricsâsuch as minimal noise and high chemical stabilityâare met or exceeded.
Applicable Materials
Section titled âApplicable MaterialsâTo achieve the cited low residual current and exceptional stability, researchers must utilize high-quality MPCVD diamond.
- Primary Material Recommendation: Heavy Boron-Doped PCD (BDD).
- Target Specifications: 6CCVD can precisely tune the boron doping level to 1000 ppm (or higher/lower, depending on the desired metallic vs. semi-metallic characteristics) required for optimal charge transfer kinetics.
- Quality Assurance: Our MPCVD growth process ensures low defect density and highly stable sp3 hybridization, critical for minimizing substrate absorption and maintaining the electrodeâs integrity across wide pH and potential ranges.
Customization Potential
Section titled âCustomization PotentialâThe required 3 mm diameter BDD electrode used in this study is a simple example of the custom dimensions 6CCVD routinely provides. We offer end-to-end material engineering support for electrochemical sensors:
| Service Category | Research Requirement Mapping | 6CCVD Capability |
|---|---|---|
| Substrate Dimensions | Small, 3 mm diameter discs | Custom laser cutting and shaping services. We supply wafers/plates up to 125 mm for bulk processing or custom sizing for single-cell analysis. |
| Film Thickness Control | Required thickness for sensor stability | Precision SCD/PCD films from 0.1 Âľm up to 500 Âľm, ensuring specific mechanical and electrical tolerances. |
| Metalization Integration | Required for ohmic contact/bonding (not explicitly detailed, but implied) | In-house capability for standard metalization schemes (Ti/Pt/Au, Ti/W/Cu, etc.) to ensure reliable electrical interface contact for sensor packaging. |
| Surface Finish | Necessary for repeatable sensor measurements | Custom polishing services: Ra < 5 nm for Inch-size PCD wafers, ensuring consistent surface morphology across the electrode working area. |
Engineering Support
Section titled âEngineering Supportâ6CCVD is committed to accelerating materials science and engineering innovations. Our in-house PhD technical team specializes in the physical and electronic properties of diamond films for demanding applications:
- Electrochemical Expertise: We assist engineers in optimizing BDD material selection (doping concentration, grain size, and surface termination) specifically for voltammetry, amperometry, and flow injection analysis projects, mirroring the cited application in chemical sensors.
- Stability and Purity: We provide consultation on maximizing BDD operational lifespan and ensuring the material achieves the low residual current necessary for nanomolar detection limits in complex media.
For custom specifications or material consultation, visit 6ccvd.com or contact our engineering team directly. We offer global shipping (DDU default, DDP available) to ensure rapid deployment of customized diamond solutions worldwide.
View Original Abstract
In this work we demonstrated electrochemistry and determination of natural compound polydatin and its quantification in tablets.Electrochemical behavior of polydatin was tested in the potential range from -1.5 to 2 V at unmodified boron doped diamond electrode in various pH of supporting electrolyte.It has been noticed that polydatin provides two oxidation peaks, one of around 0.73 and second at around 1.3 V.In the reverse scan no reduction peak was observed.Quantification of polydatin was done based on first oxidation peak using square wave voltammetry.After optimization of the method linear working range from 1â˘10-7 to 7â˘10-5 M was obtained, with limit of detection and limit of quantification of 6â˘10-9 M and 2â˘10-8 M, respectively.Negligible interferences effect was noted.Developed method shows excellent accuracy and precision toward detection of polydatin and was used for its quantification in the pharmaceutical samples.