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Realization and Characterization of Carbonic Layers on 4H-SiC for Electrochemical Detections

MetadataDetails
Publication Date2017-05-15
JournalMaterials science forum
AuthorsJulien Pézard, Véronique SouliÚre, Mihai Lazar, Naoufel Haddour, François Buret
InstitutionsInstitut National des Sciences Appliquées de Lyon, Laboratoire des Multimatériaux et Interfaces
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Technical Analysis and Material Sourcing Documentation: Carbonic Layers on SiC for Electrochemical Sensing

Section titled “Technical Analysis and Material Sourcing Documentation: Carbonic Layers on SiC for Electrochemical Sensing”

Document Reference: Realization and characterization of carbonic layers on 4H-SiC for electrochemical detections (Pezard et al.) Prepared For: Engineering and Scientific Clients Date: October 26, 2023 Prepared By: 6CCVD Technical Engineering Team


This research successfully demonstrates the integration of multiple advanced carbon thin films—specifically Boron Doped Diamond (BDD), Graphene, and Pyrolyzed Photoresist Films (PPF)—onto 4H-SiC substrates for high-performance electrochemical biosensors. This approach aligns with the industry trend toward fully integrated, robust sensing platforms.

  • Core Achievement: Successful synthesis of high-quality BDD films on 4H-SiC using Plasma-Assisted Chemical Vapour Deposition (PACVD), demonstrating feasibility for large-scale SiC technology integration.
  • Performance Metrics: BDD electrodes exhibited high sensitivity (12.83 ”A/mM) and broad potential windows, confirming superior electroactivity compared to traditional glassy carbon electrodes.
  • Biosensor Application: The BDD transducers were successfully functionalized with acetylthiocholinesterase for the detection of acetylthiocholine, yielding a clinically relevant limit of detection (22.8 ”M).
  • Integration Potential: All carbon layers were shown to be compatible with high-temperature processing (up to 1650 °C) and possess high patternability, crucial for transitioning from macrometric to micrometric integrated devices.
  • Material Strength: The intrinsic chemical robustness and reproducibility of BDD films make them ideal for long-term, high-reliability biosensing applications where resistance to high temperatures and aggressive environments is critical.

The table below summarizes the critical electrochemical performance data for the Boron Doped Diamond (BDD) electrodes, essential for replicating or extending this research.

ParameterValueUnitContext
Synthesis Method (BDD)PACVDN/ADeposition on 4H-SiC substrate
Electrode Area Tested1cm2Sample size for biosensor evaluation
BDD Sensitivity12.83 ±0.44”A/mMHighest sensitivity achieved among the three carbon films for acetylthiocholine detection
Limit of Detection (LOD)2.28 E-05M (22.8 ”M)Lowest concentration detected (Diamond)
Linear Range2.28 E-05 to 0.45 E-03M (22.8 ”M to 0.45 mM)Effective measurement range (Diamond)
Maximum Reaction Speed (Imax)8.31 E-06 ±2.08E-08AChronoamperometry result (Diamond)
Michaelis Menten Constant (KM)0.23 ±5.75 E-04mMEnzyme affinity/activity (Diamond)
Potential Window (KCl 0.1M)-1 to -1.37VElectroactivity range (Diamond)
Potential Window (PBS 10X)-1.1 to -1.25VElectroactivity range (Diamond)
Graphene Synthesis Temperature1550°CSilicon sublimation on SiC in Ar atmosphere
PPF Maximum Annealing Temperature1650°COptimized for maximum electrical conductivity

The synthesis of the carbon films required specific high-temperature and vacuum processing steps, demonstrating compatibility with SiC fabrication technologies.

  1. Substrate Preparation: Commercial 4H-SiC substrates featuring a low-doped n-type epilayer were used to isolate the electrochemical response of the carbon films from the substrate.
  2. Boron Doped Diamond (BDD) Deposition: BDD films were grown directly onto the SiC substrates using Plasma-Assisted Chemical Vapour Deposition (PACVD), yielding conductive, heavily boron-doped layers necessary for electrochemical activity.
  3. Pyrolyzed Photoresist Film (PPF) Synthesis:
    • A 1 ”m thick AZ5214E photoresist layer was applied via spin coating.
    • Annealing Step 1 (Pyrolysis): Primary pyrolysis was conducted under primary vacuum at 750 °C for 30 minutes.
    • Annealing Step 2 (Graphitization): A second high-temperature annealing step was performed using an induction Rapid Thermal Annealing (RTA) furnace for 30 minutes, under Argon (Ar) gas, with temperature plateaus varied between 1150 °C and 1650 °C.
  4. Graphene Synthesis: Graphene was grown via Silicon sublimation from the SiC surface at 1550 °C under an Ar atmosphere, utilizing the same induction RTA furnace as the PPF process.
  5. Characterization: Four-point probe measurements determined conductivity. Electrochemical properties were studied using potentiostat characterization. Layer thickness was measured via alpha-step profilometer, and structural changes were analyzed via ”-Raman (633 nm HeNe laser).
  6. Biosensor Functionalization: Electrodes (1 cm2 samples) were functionalized for acetylthiocholine detection by adsorption using a 0.5% chitosan solution in acetic acid (pH 5).

6CCVD Solutions & Capabilities: Enabling Integrated Diamond Biosensors

Section titled “6CCVD Solutions & Capabilities: Enabling Integrated Diamond Biosensors”

This research confirms the potential of Boron Doped Diamond (BDD) as a transducer material for high-sensitivity, integrated electrochemical sensors compatible with SiC technology. 6CCVD is an industry leader in manufacturing high-quality MPCVD Diamond, offering materials and engineering services perfectly matched to replicate, scale, and optimize this work.

To meet the high-performance and structural integration requirements outlined in the paper, 6CCVD strongly recommends the following materials:

6CCVD Material SpecificationApplication Match & Technical Advantage
Heavy Boron Doped PCD (BDD)Directly addresses the need for conductive diamond electrodes. Our heavily doped polycrystalline diamond (PCD) films offer the high signal-to-noise ratio and wide potential range required for robust electrochemistry, exceeding standard glassy carbon performance.
PCD Thin Films (0.1”m - 500”m)Provides the necessary thinness for integrated devices. Our PCD can be grown on customer-supplied substrates (e.g., 4H-SiC wafers) to guarantee compatibility with existing SiC processing steps.
Optical Grade SCD SubstratesWhile this paper used BDD on SiC, our high-purity SCD substrates (up to 10mm thickness) offer unparalleled thermal and chemical stability for highly sensitive applications demanding the lowest background noise or requiring optical transparency in the setup.

The paper’s conclusion stresses the need to transition electrodes from macrometric to micrometric dimensions for all-inclusive devices. 6CCVD’s specialized fabrication services are critical for achieving this next step:

  • Custom Dimensions and Etching: 6CCVD offers high-precision laser micro-cutting and shaping services. We can produce complex electrode patterns and micro-structures down to the micron level on BDD films grown on up to 125mm wafers, perfectly suited for integrated SiC micro-systems.
  • Custom Thickness Control: We offer precise control over BDD film thickness, ranging from 0.1 ”m to 500 ”m, allowing researchers to tune the electrode properties (conductivity, mechanical strength) as a function of device integration requirements.
  • Metalization Services: Although not the focus of this specific paper, electrochemical sensors often require reliable electrical contacts. 6CCVD provides in-house multi-layer metalization capabilities (including Ti, Pt, Au, Pd, W, and Cu) applied directly to the diamond surface, ensuring robust contacts compatible with micro-device assembly.
  • High-Quality Polishing: For applications requiring high-resolution imaging or highly reproducible surface chemistry, 6CCVD can polish large-area PCD/BDD wafers (up to inch-size) to an Ra < 5 nm finish, and Single Crystal Diamond (SCD) surfaces to Ra < 1 nm.

Our commitment extends beyond material supply. 6CCVD’s in-house PhD team, specializing in MPCVD synthesis and diamond characterization, can assist with material selection and deposition parameter optimization for similar SiC-integrated biosensing or high-temperature electrochemical projects.

We ensure seamless global procurement logistics. All 6CCVD materials are shipped Globally (DDU default, DDP available), providing reliable, secure delivery of sensitive materials worldwide.


For custom specifications or material consultation, visit 6ccvd.com or contact our engineering team directly. We are ready to support your transition from lab-scale prototypes to fully integrated diamond micro-devices.

View Original Abstract

Carbon materials are considered building blocks for most of electrochemical sensors. Their biocompatibility allows their use as transducers for biosensors. Furthermore, they can be patterned, giving interest in all-inclusive bioelectrochemical detection devices. In particular, graphene, boron doped diamond, and pyrolyzed photoresist films are suitable for this kind of application, which would give the ability to use these working electrodes within a fully designed apparatus completed by counter and reference electrode. In this paper, a pioneer work is exposed on the synthesis of these materials for use as electrochemical sensors and as transducers for biodetection of acetylcholine by adsorption of acetylthiocholinesterase. The lowest limit of detection reaches 6.98 ÎŒM, sensitivity 5.91 ÎŒA/mM, and a linear range from 6.98 ÎŒM to 0.55 mM.