Fiber-optic electron-spin-resonance thermometry of single laser-activated neurons
At a Glance
Section titled āAt a Glanceā| Metadata | Details |
|---|---|
| Publication Date | 2016-11-28 |
| Journal | Optics Letters |
| Authors | A. Š. Lanin, Š. Š. Š¤ŠµŠ“Š¾ŃŠ¾Š², Yulia G. Ermakova, D. A. SidorovāBiryukov, A. B. Fedotov |
| Institutions | Institute of Bioorganic Chemistry, Texas A&M University |
| Citations | 35 |
Abstract
Section titled āAbstractāOptically detected electron spin resonance in fiber-coupled nitrogen-vacancy (NV) centers of diamond is used to demonstrate a fiber-optic quantum thermometry of individual thermogenetically activated neurons. Laser-induced temperature variations read out from single neurons with the NV-diamond fiber sensor are shown to strongly correlate with the fluorescence of calcium-ion sensors, serving as online indicators of the inward Ca<sup>2+</sup> current across the cell membrane of neurons expressing transient receptor potential (TRP) cation channels. Local laser heating above the TRP-channel activation threshold is shown to reproducibly evoke robust action potentials, visualized by calcium-ion-sensor-aided fluorescence imaging and detected as prominent characteristic waveforms in the time-resolved response of fluorescence Ca<sup>2+</sup> sensors.